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Registration services to Thai FDA for cosmetics and food supplements

Compounds/Herbs analysis

  1. Compounds/Herbs analysed by HPLC and UV-detector
  2. Compounds/Herbs analysed by HPLC and refractive index detector
  3. Compounds/Herbs analysed by UV-visible spectrophotometer
  4. DNA/RNA analysis by agarose gel electrophoresis
  5. Proteins and peptides analysed by polyacrylamide gel electrophoresis
  6. Protein analysis
  7. Phytochemicals in herbs (alkaloids, anthraquinones, carotenoids, glycosides, xanthones, flavones, tannins and triterpenes/steroids) analysed by chemical method

Biological activities of compounds/herbs

  1. Anti-proliferative activity on cancer cell lines assayed by sulforhodamine B (SRB) assay :
  • Mouth epidermal carcinoma cells (KB cells)
  • Liver hepatocellular carcinoma cells (HepG2 cells) 1.3 Colon adenocarcinoma cells (HT29 cells)
  • Cervix adenocarcinoma cells (HeLa cells)
  • Prostate carcinoma cells (DU145 cells)
  • Lung carcinoma cells (A549 cells)
  • Others

  2.  Anti-bacterial activity of compounds/herbs assayed by the disc diffusion method :

  • Staphylococcus aureus
  • Streptococcus mutans
  • Propionibacterium acnes
  • Others
  3.  Apoptosis activity assayed by Acridine Orange / Ethidium Bromide (AO/EB)

  4.  In vitro immune-stimulating activity assayed by Nitroblue Tetrazolium Test (NBT)

  5.  Matrix metalloproteinase-2 (enzymatic degradation of collagen) stimulating/inhibiting activity assayed by   gelatin zymography

  6.  Proteolytic activity assayed by spectrophotometer

  7.  In vitro tyrosinase inhibitory activity assayed by spectrophotometer

  8.  Angiotensin Converting Enzyme (ACE) inhibiting activity assayed by kits

  9.  Anti-oxidation activity assay

  • Free-radical scavenging by DPPH method
  • Lipid peroxidation inhibition by ferric iron-thiocyanate complex method
  • Metal chelating by ferrous iron-ferrozine complex method

 10.  In vivo anti-cancer activity in human tumor xenograft nude mice

 11.   Anti-hypertension activity assay by tail cuff in mice

 12.  Anti-diabetes activity assay in alloxan-induced diabetes mice

 13.  5α reductase inhibiting activity assay (for anti-hair loss)

 14.  Melanogenesis activity assay (for hair / skin darkening)

 15.  Others

Quality and safety of compounds/herbs determination
  1. Transdermal absorption through skin by Franz Diffusion Cell
  2. Skin irritation by closed patch test on rabbit’s skin
  3. Bacterial contamination (Escherichia coli, Salmonella spp. and aerobic bacteria) by
  4. spread-plate technique
  5. Cytotoxicity in human skin fibroblast by sulforhodamine B (SRB) assay
  6. Acute / subacute / subchronic toxicity in mice and rats

Development of extracts from herbal and natural products 

Development of formulations containing compounds and natural extracts

  1. nanoparticles / liposomes / niosomes / cubosomes
  2. tablet / capsule / granule / tea
  3. cream / gel / lotion
  4. Others

Consultancy and researches of raw materials and products for health and beauty for commercialization

Otherservices:

  1. Genetic material analysis
  2. Biopharmacy
  3. Agricultural science
  4. Food analysis
  5. Product analysis
  6. Environmental analysis
  7. Product registration to Food and Drug Administration
  8. OH group and double bond addition by biotechnology
  9. Compound production by Bioconversion/Fermentation
  10. DNA transformation and transfection

Examples of equipments and instruments for researches, development and analysis